This guide provides comprehensive steps for operating a spectrometer and understanding its results. A spectrometer is an analytical tool used across various scientific disciplines to measure how a substance interacts with light. Specifically, a UV-Visible Spectrometer measures the absorption or transmission of light in the ultraviolet (UV) and visible (Vis) regions of the electromagnetic. A spectrophotometer is an instrument used to measure the intensity of light absorbed by a sample at different wavelengths. It functions by separating light into its constituent wavelengths, much like a prism splits sunlight into a rainbow. It is a crucial tool in various fields, including chemistry, biology, and environmental science.
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